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pbpD [2018-02-16 18:25:08]
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pbpD [2018-02-16 18:25:08]

penicillin-binding protein 4
Locus
BSU31490
Isoelectric point
9.31
Molecular weight
70.45 kDa
Protein length
624 aa Sequence Blast
Gene length
1872 bp Sequence Blast
Function
bifunctional glucosyltransferase/ transpeptidase
Product
penicillin-binding protein 4
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Cell envelope and cell division → Cell wall synthesis → Penicillin-binding proteins
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    3,233,911 → 3,235,785

    The protein

    Paralogous protein(s)

  • PbpF, PbpG, PonA

    • Structure

  • 3VMA (PBP1B from E. coli, 28% identity) PubMed

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    pbpD-yuxK
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Additional information

  • the terminator is NusA-dependent [ http://www.nature.com/articles/nmicrobiol20157 Reference]
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    Biological materials

    Mutant

  • BKE31490 (ΔpbpD::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTCTCTTTTCTCCTAAAT, downstream forward: _UP4_TAAAGGATCGGAGGGGATAT
  • BKK31490 (ΔpbpD::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTCTCTTTTCTCCTAAAT, downstream forward: _UP4_TAAAGGATCGGAGGGGATAT

    • GFP fusion

  • 2082 trpC2 pbpD::pSG1490 (cat Pxyl-gfpa-pbpD1-510) PubMed, available in Dirk Jan Scheffers' lab and in the BGSC

    • References

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